Construct: shRNA TRCN0000440082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005475.1-2808s21c1
DNA Barcode:
AGTAGTCTGGAGGACACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Iqsec2 (245666)
Gene Description:
IQ motif and Sec7 domain 2
Transcript:
RefSeq NM_001005475.1 (NON-CURRENT)
Match location:
Position 2808 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGTCTGGAGGACACTTAT
Hairpin Sequence:
5'-CCGG-AGTAGTCTGGAGGACACTTAT-CTCGAG-ATAAGTGTCCTCCAGACTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGTCTGGAGGACACTTATCTCGAGATAAGTGTCCTCCAGACTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGTCTGGAGGACACTTATCTCGAGATAAGTGTCCTCCAGACTACT-3'

Other clones with same target sequence:

(none)