Construct: shRNA TRCN0000440384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173475.2-1477s21c1
DNA Barcode:
CAGATCTCAGTTTCGCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCUN1D3 (123879)
Gene Description:
defective in cullin neddylation 1 domain containing 3
Transcript:
RefSeq NM_173475.2 (NON-CURRENT)
Match location:
Position 1477 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATCTCAGTTTCGCATATT
Hairpin Sequence:
5'-CCGG-CAGATCTCAGTTTCGCATATT-CTCGAG-AATATGCGAAACTGAGATCTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATCTCAGTTTCGCATATTCTCGAGAATATGCGAAACTGAGATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATCTCAGTTTCGCATATTCTCGAGAATATGCGAAACTGAGATCTG-3'

Other clones with same target sequence:

(none)