Construct: shRNA TRCN0000440501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021822.2-581s21c1
DNA Barcode:
TGCATCGTGACCAGGAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOBEC3G (60489)
Gene Description:
apolipoprotein B mRNA editing enzyme catalytic subunit 3G
Transcript:
RefSeq NM_021822.2 (NON-CURRENT)
Match location:
Position 581 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCATCGTGACCAGGAGTATG
Hairpin Sequence:
5'-CCGG-TGCATCGTGACCAGGAGTATG-CTCGAG-CATACTCCTGGTCACGATGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCATCGTGACCAGGAGTATGCTCGAGCATACTCCTGGTCACGATGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCATCGTGACCAGGAGTATGCTCGAGCATACTCCTGGTCACGATGCA-3'

Other clones with same target sequence:

(none)