Construct: shRNA TRCN0000440885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153331.3-1055s21c1
DNA Barcode:
GTGGAGCACAACTGGACTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCTD6 (200845)
Gene Description:
potassium channel tetramerization domain containing 6
Transcript:
RefSeq NM_153331.3 (CURRENT)
Match location:
Position 1055 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAGCACAACTGGACTTTC
Hairpin Sequence:
5'-CCGG-GTGGAGCACAACTGGACTTTC-CTCGAG-GAAAGTCCAGTTGTGCTCCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAGCACAACTGGACTTTCCTCGAGGAAAGTCCAGTTGTGCTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAGCACAACTGGACTTTCCTCGAGGAAAGTCCAGTTGTGCTCCAC-3'

Other clones with same target sequence:

(none)