Construct: shRNA TRCN0000440933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021013.3-1466s21c1
DNA Barcode:
GCTTTCCTGACCCAAGCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT34 (3885)
Gene Description:
keratin 34
Transcript:
RefSeq NM_021013.3 (CURRENT)
Match location:
Position 1466 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCCTGACCCAAGCAAAG
Hairpin Sequence:
5'-CCGG-GCTTTCCTGACCCAAGCAAAG-CTCGAG-CTTTGCTTGGGTCAGGAAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCCTGACCCAAGCAAAGCTCGAGCTTTGCTTGGGTCAGGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCCTGACCCAAGCAAAGCTCGAGCTTTGCTTGGGTCAGGAAAGC-3'

Other clones with same target sequence:

(none)