Construct: shRNA TRCN0000440945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000667.2-818s21c1
DNA Barcode:
ACCCATCCAGGAGGTGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADH1A (124)
Gene Description:
alcohol dehydrogenase 1A (class I), alpha polypeptide
Transcript:
RefSeq NM_000667.2 (NON-CURRENT)
Match location:
Position 818 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCATCCAGGAGGTGCTAAA
Hairpin Sequence:
5'-CCGG-ACCCATCCAGGAGGTGCTAAA-CTCGAG-TTTAGCACCTCCTGGATGGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCATCCAGGAGGTGCTAAACTCGAGTTTAGCACCTCCTGGATGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCATCCAGGAGGTGCTAAACTCGAGTTTAGCACCTCCTGGATGGGT-3'

Other clones with same target sequence:

(none)