Construct: shRNA TRCN0000440993

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145235.3-878s21c1
DNA Barcode:
GAGGGTGGCCTCTCTTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FANK1 (92565)
Gene Description:
fibronectin type III and ankyrin repeat domains 1
Transcript:
RefSeq NM_145235.3 (NON-CURRENT)
Match location:
Position 878 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440993)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGTGGCCTCTCTTCTAAT
Hairpin Sequence:
5'-CCGG-GAGGGTGGCCTCTCTTCTAAT-CTCGAG-ATTAGAAGAGAGGCCACCCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGTGGCCTCTCTTCTAATCTCGAGATTAGAAGAGAGGCCACCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGTGGCCTCTCTTCTAATCTCGAGATTAGAAGAGAGGCCACCCTC-3'

Other clones with same target sequence:

(none)