Construct: shRNA TRCN0000441448

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000727.2-1081s21c1
DNA Barcode:
TGCAGGGACAACCTGTGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNG1 (786)
Gene Description:
calcium voltage-gated channel auxiliary subunit gamma 1
Transcript:
RefSeq NM_000727.2 (NON-CURRENT)
Match location:
Position 1081 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000441448)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCAGGGACAACCTGTGTTTG
Hairpin Sequence:
5'-CCGG-TGCAGGGACAACCTGTGTTTG-CTCGAG-CAAACACAGGTTGTCCCTGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCAGGGACAACCTGTGTTTGCTCGAGCAAACACAGGTTGTCCCTGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCAGGGACAACCTGTGTTTGCTCGAGCAAACACAGGTTGTCCCTGCA-3'

Other clones with same target sequence:

(none)