Construct: shRNA TRCN0000441502

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010590.4-488s21c1
DNA Barcode:
TCTGTCGTCGCCTCCTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ajuba (16475)
Gene Description:
ajuba LIM protein
Transcript:
RefSeq NM_010590.4 (NON-CURRENT)
Match location:
Position 488 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000441502)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGTCGTCGCCTCCTGATTT
Hairpin Sequence:
5'-CCGG-TCTGTCGTCGCCTCCTGATTT-CTCGAG-AAATCAGGAGGCGACGACAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGTCGTCGCCTCCTGATTTCTCGAGAAATCAGGAGGCGACGACAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGTCGTCGCCTCCTGATTTCTCGAGAAATCAGGAGGCGACGACAGA-3'

Other clones with same target sequence:

(none)