Construct: shRNA TRCN0000441967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000036.2-1919s21c1
DNA Barcode:
ATATCTCTCATGGCCTAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMPD1 (270)
Gene Description:
adenosine monophosphate deaminase 1
Transcript:
RefSeq NM_000036.2 (CURRENT)
Match location:
Position 1919 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000441967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATCTCTCATGGCCTAAATT
Hairpin Sequence:
5'-CCGG-ATATCTCTCATGGCCTAAATT-CTCGAG-AATTTAGGCCATGAGAGATAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATCTCTCATGGCCTAAATTCTCGAGAATTTAGGCCATGAGAGATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATCTCTCATGGCCTAAATTCTCGAGAATTTAGGCCATGAGAGATAT-3'

Other clones with same target sequence:

(none)