Construct: shRNA TRCN0000442166
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013661.2-446s21c1
- DNA Barcode:
- ATCTGAAGCCGTGGGTCTTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sema5b (20357)
- Gene Description:
- sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
- Transcript:
- RefSeq NM_013661.2 (CURRENT)
- Match location:
- Position 446 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000442166)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ATCTGAAGCCGTGGGTCTTTA
- Hairpin Sequence:
-
5'-CCGG-ATCTGAAGCCGTGGGTCTTTA-CTCGAG-TAAAGACCCACGGCTTCAGAT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGATCTGAAGCCGTGGGTCTTTACTCGAGTAAAGACCCACGGCTTCAGATTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAATCTGAAGCCGTGGGTCTTTACTCGAGTAAAGACCCACGGCTTCAGAT-3'
Other clones with same target sequence:
(none)
