Construct: shRNA TRCN0000442182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145533.1-1464s21c1
DNA Barcode:
CACAGAGATGCTTCGACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smox (228608)
Gene Description:
spermine oxidase
Transcript:
RefSeq NM_145533.1 (NON-CURRENT)
Match location:
Position 1464 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGAGATGCTTCGACAGTT
Hairpin Sequence:
5'-CCGG-CACAGAGATGCTTCGACAGTT-CTCGAG-AACTGTCGAAGCATCTCTGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGAGATGCTTCGACAGTTCTCGAGAACTGTCGAAGCATCTCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGAGATGCTTCGACAGTTCTCGAGAACTGTCGAAGCATCTCTGTG-3'

Other clones with same target sequence:

(none)