Construct: shRNA TRCN0000442209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130888.1-1140s21c1
DNA Barcode:
TATGGGTTCAGAGGTGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nxf7 (170722)
Gene Description:
nuclear RNA export factor 7
Transcript:
RefSeq NM_130888.1 (CURRENT)
Match location:
Position 1140 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGGGTTCAGAGGTGATAAA
Hairpin Sequence:
5'-CCGG-TATGGGTTCAGAGGTGATAAA-CTCGAG-TTTATCACCTCTGAACCCATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGGGTTCAGAGGTGATAAACTCGAGTTTATCACCTCTGAACCCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGGGTTCAGAGGTGATAAACTCGAGTTTATCACCTCTGAACCCATA-3'

Other clones with same target sequence:

(none)