Construct: shRNA TRCN0000442347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057176.2-338s21c1
DNA Barcode:
AGGTCTACGGCACCTTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BSND (7809)
Gene Description:
barttin CLCNK type accessory beta subunit
Transcript:
RefSeq NM_057176.2 (NON-CURRENT)
Match location:
Position 338 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGTCTACGGCACCTTCTATG
Hairpin Sequence:
5'-CCGG-AGGTCTACGGCACCTTCTATG-CTCGAG-CATAGAAGGTGCCGTAGACCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGTCTACGGCACCTTCTATGCTCGAGCATAGAAGGTGCCGTAGACCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGTCTACGGCACCTTCTATGCTCGAGCATAGAAGGTGCCGTAGACCT-3'

Other clones with same target sequence:

(none)