Construct: shRNA TRCN0000442592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001134461.1-2407s21c1
DNA Barcode:
CTACTTCACGGAGTATGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prickle2 (243548)
Gene Description:
prickle planar cell polarity protein 2
Transcript:
RefSeq NM_001134461.1 (CURRENT)
Match location:
Position 2407 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTTCACGGAGTATGATTG
Hairpin Sequence:
5'-CCGG-CTACTTCACGGAGTATGATTG-CTCGAG-CAATCATACTCCGTGAAGTAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTTCACGGAGTATGATTGCTCGAGCAATCATACTCCGTGAAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTTCACGGAGTATGATTGCTCGAGCAATCATACTCCGTGAAGTAG-3'

Other clones with same target sequence:

(none)