Construct: shRNA TRCN0000442905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001850.3-278s21c1
DNA Barcode:
GGGAGTGCTGCTTACCATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL8A1 (1295)
Gene Description:
collagen type VIII alpha 1 chain
Transcript:
RefSeq NM_001850.3 (NON-CURRENT)
Match location:
Position 278 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGAGTGCTGCTTACCATTTC
Hairpin Sequence:
5'-CCGG-GGGAGTGCTGCTTACCATTTC-CTCGAG-GAAATGGTAAGCAGCACTCCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGAGTGCTGCTTACCATTTCCTCGAGGAAATGGTAAGCAGCACTCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGAGTGCTGCTTACCATTTCCTCGAGGAAATGGTAAGCAGCACTCCC-3'

Other clones with same target sequence:

(none)