Construct: shRNA TRCN0000442916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018240.5-1765s21c1
DNA Barcode:
CCACGCTCACCATCAACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIRREL1 (55243)
Gene Description:
kirre like nephrin family adhesion molecule 1
Transcript:
RefSeq NM_018240.5 (NON-CURRENT)
Match location:
Position 1765 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGCTCACCATCAACAATG
Hairpin Sequence:
5'-CCGG-CCACGCTCACCATCAACAATG-CTCGAG-CATTGTTGATGGTGAGCGTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGCTCACCATCAACAATGCTCGAGCATTGTTGATGGTGAGCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGCTCACCATCAACAATGCTCGAGCATTGTTGATGGTGAGCGTGG-3'

Other clones with same target sequence:

(none)