Construct: shRNA TRCN0000443029

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028450.3-1448s21c1
DNA Barcode:
CCTTGACATTTATGCTCATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gulp1 (70676)
Gene Description:
GULP, engulfment adaptor PTB domain containing 1
Transcript:
RefSeq NM_028450.3 (CURRENT)
Match location:
Position 1448 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443029)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGACATTTATGCTCATTC
Hairpin Sequence:
5'-CCGG-CCTTGACATTTATGCTCATTC-CTCGAG-GAATGAGCATAAATGTCAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGACATTTATGCTCATTCCTCGAGGAATGAGCATAAATGTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGACATTTATGCTCATTCCTCGAGGAATGAGCATAAATGTCAAGG-3'

Other clones with same target sequence:

(none)