Construct: shRNA TRCN0000443255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_213615.2-1403s21c1
DNA Barcode:
TATAGTGGAAAGCACAAATCC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A530032D15Rik (381287)
Gene Description:
RIKEN cDNA A530032D15Rik gene
Transcript:
RefSeq NM_213615.2 (CURRENT)
Match location:
Position 1403 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATAGTGGAAAGCACAAATCC
Hairpin Sequence:
5'-CCGG-TATAGTGGAAAGCACAAATCC-CTCGAG-GGATTTGTGCTTTCCACTATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATAGTGGAAAGCACAAATCCCTCGAGGGATTTGTGCTTTCCACTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATAGTGGAAAGCACAAATCCCTCGAGGGATTTGTGCTTTCCACTATA-3'

Other clones with same target sequence:

(none)