Construct: shRNA TRCN0000443340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177533.3-256s21c1
DNA Barcode:
TTCAACTCTTCTCGGAGGAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDT14 (256281)
Gene Description:
nudix hydrolase 14
Transcript:
RefSeq NM_177533.3 (NON-CURRENT)
Match location:
Position 256 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAACTCTTCTCGGAGGAGC
Hairpin Sequence:
5'-CCGG-TTCAACTCTTCTCGGAGGAGC-CTCGAG-GCTCCTCCGAGAAGAGTTGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAACTCTTCTCGGAGGAGCCTCGAGGCTCCTCCGAGAAGAGTTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAACTCTTCTCGGAGGAGCCTCGAGGCTCCTCCGAGAAGAGTTGAA-3'

Other clones with same target sequence:

(none)