Construct: shRNA TRCN0000443560

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028986.3-1700s21c1
DNA Barcode:
CAATTCCCTGTACCAGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gzf1 (74533)
Gene Description:
GDNF-inducible zinc finger protein 1
Transcript:
RefSeq NM_028986.3 (CURRENT)
Match location:
Position 1700 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443560)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTCCCTGTACCAGCATAT
Hairpin Sequence:
5'-CCGG-CAATTCCCTGTACCAGCATAT-CTCGAG-ATATGCTGGTACAGGGAATTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTCCCTGTACCAGCATATCTCGAGATATGCTGGTACAGGGAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTCCCTGTACCAGCATATCTCGAGATATGCTGGTACAGGGAATTG-3'

Other clones with same target sequence:

(none)