Construct: shRNA TRCN0000443915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_205862.1-550s21c1
DNA Barcode:
GCAAAGCGCATGGAGACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A6 (54578)
Gene Description:
UDP glucuronosyltransferase family 1 member A6
Transcript:
RefSeq NM_205862.1 (NON-CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGCGCATGGAGACTAAG
Hairpin Sequence:
5'-CCGG-GCAAAGCGCATGGAGACTAAG-CTCGAG-CTTAGTCTCCATGCGCTTTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGCGCATGGAGACTAAGCTCGAGCTTAGTCTCCATGCGCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGCGCATGGAGACTAAGCTCGAGCTTAGTCTCCATGCGCTTTGC-3'

Other clones with same target sequence:

(none)