Construct: shRNA TRCN0000443938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005125.1-990s21c1
DNA Barcode:
GTGGTGTTCCCTTGGCAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCS (9973)
Gene Description:
copper chaperone for superoxide dismutase
Transcript:
RefSeq NM_005125.1 (NON-CURRENT)
Match location:
Position 990 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTGTTCCCTTGGCAAATG
Hairpin Sequence:
5'-CCGG-GTGGTGTTCCCTTGGCAAATG-CTCGAG-CATTTGCCAAGGGAACACCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTGTTCCCTTGGCAAATGCTCGAGCATTTGCCAAGGGAACACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTGTTCCCTTGGCAAATGCTCGAGCATTTGCCAAGGGAACACCAC-3'

Other clones with same target sequence:

(none)