Construct: shRNA TRCN0000443946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012201.5-2498s21c1
DNA Barcode:
CCGCTTGGAGCCAGATCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLG1 (2734)
Gene Description:
golgi glycoprotein 1
Transcript:
RefSeq NM_012201.5 (NON-CURRENT)
Match location:
Position 2498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000443946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCTTGGAGCCAGATCTATA
Hairpin Sequence:
5'-CCGG-CCGCTTGGAGCCAGATCTATA-CTCGAG-TATAGATCTGGCTCCAAGCGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCTTGGAGCCAGATCTATACTCGAGTATAGATCTGGCTCCAAGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCTTGGAGCCAGATCTATACTCGAGTATAGATCTGGCTCCAAGCGG-3'

Other clones with same target sequence:

(none)