Construct: shRNA TRCN0000444028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001010985.2-1128s21c1
DNA Barcode:
AGGCATGGTACTCTGACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYBPHL (343263)
Gene Description:
myosin binding protein H like
Transcript:
RefSeq NM_001010985.2 (NON-CURRENT)
Match location:
Position 1128 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCATGGTACTCTGACAAAG
Hairpin Sequence:
5'-CCGG-AGGCATGGTACTCTGACAAAG-CTCGAG-CTTTGTCAGAGTACCATGCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCATGGTACTCTGACAAAGCTCGAGCTTTGTCAGAGTACCATGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCATGGTACTCTGACAAAGCTCGAGCTTTGTCAGAGTACCATGCCT-3'

Other clones with same target sequence:

(none)