Construct: shRNA TRCN0000444288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013399.2-598s21c1
DNA Barcode:
TGCTGCAGGGAGAGATCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDIP1 (29965)
Gene Description:
cell death inducing p53 target 1
Transcript:
RefSeq NM_013399.2 (NON-CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGCAGGGAGAGATCTTTG
Hairpin Sequence:
5'-CCGG-TGCTGCAGGGAGAGATCTTTG-CTCGAG-CAAAGATCTCTCCCTGCAGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGCAGGGAGAGATCTTTGCTCGAGCAAAGATCTCTCCCTGCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGCAGGGAGAGATCTTTGCTCGAGCAAAGATCTCTCCCTGCAGCA-3'

Other clones with same target sequence:

(none)