Construct: shRNA TRCN0000444319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005742.2-875s21c1
DNA Barcode:
GCGAGTCTCCTGTGGATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDIA6 (10130)
Gene Description:
protein disulfide isomerase family A member 6
Transcript:
RefSeq NM_005742.2 (NON-CURRENT)
Match location:
Position 875 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGTCTCCTGTGGATTATG
Hairpin Sequence:
5'-CCGG-GCGAGTCTCCTGTGGATTATG-CTCGAG-CATAATCCACAGGAGACTCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGTCTCCTGTGGATTATGCTCGAGCATAATCCACAGGAGACTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGTCTCCTGTGGATTATGCTCGAGCATAATCCACAGGAGACTCGC-3'

Other clones with same target sequence:

(none)