Construct: shRNA TRCN0000444439

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003023.4-1622s21c1
DNA Barcode:
CCAACTCGGTCTTCGTCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3BP2 (6452)
Gene Description:
SH3 domain binding protein 2
Transcript:
RefSeq NM_003023.4 (CURRENT)
Match location:
Position 1622 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444439)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACTCGGTCTTCGTCAACA
Hairpin Sequence:
5'-CCGG-CCAACTCGGTCTTCGTCAACA-CTCGAG-TGTTGACGAAGACCGAGTTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACTCGGTCTTCGTCAACACTCGAGTGTTGACGAAGACCGAGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACTCGGTCTTCGTCAACACTCGAGTGTTGACGAAGACCGAGTTGG-3'

Other clones with same target sequence:

(none)