Construct: shRNA TRCN0000444488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003527.4-209s21c1
DNA Barcode:
GAAGGCCATGGGCATCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2BC17 (8348)
Gene Description:
H2B clustered histone 17
Transcript:
RefSeq NM_003527.4 (CURRENT)
Match location:
Position 209 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGCCATGGGCATCATGAA
Hairpin Sequence:
5'-CCGG-GAAGGCCATGGGCATCATGAA-CTCGAG-TTCATGATGCCCATGGCCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGCCATGGGCATCATGAACTCGAGTTCATGATGCCCATGGCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGCCATGGGCATCATGAACTCGAGTTCATGATGCCCATGGCCTTC-3'

Other clones with same target sequence:

(none)