Construct: shRNA TRCN0000444496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024108.1-445s21c1
DNA Barcode:
TATACCCTGGGCATTGAGAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAPPC6A (79090)
Gene Description:
trafficking protein particle complex 6A
Transcript:
RefSeq NM_024108.1 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATACCCTGGGCATTGAGAGC
Hairpin Sequence:
5'-CCGG-TATACCCTGGGCATTGAGAGC-CTCGAG-GCTCTCAATGCCCAGGGTATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATACCCTGGGCATTGAGAGCCTCGAGGCTCTCAATGCCCAGGGTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATACCCTGGGCATTGAGAGCCTCGAGGCTCTCAATGCCCAGGGTATA-3'

Other clones with same target sequence:

(none)