Construct: shRNA TRCN0000444848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009389.2-3012s21c1
DNA Barcode:
TACGTGAAGGACGGCAGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tle3 (21887)
Gene Description:
transducin-like enhancer of split 3
Transcript:
RefSeq NM_009389.2 (CURRENT)
Match location:
Position 3012 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGTGAAGGACGGCAGTTAC
Hairpin Sequence:
5'-CCGG-TACGTGAAGGACGGCAGTTAC-CTCGAG-GTAACTGCCGTCCTTCACGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGTGAAGGACGGCAGTTACCTCGAGGTAACTGCCGTCCTTCACGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGTGAAGGACGGCAGTTACCTCGAGGTAACTGCCGTCCTTCACGTA-3'

Other clones with same target sequence:

(none)