Construct: shRNA TRCN0000445022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020988.2-1547s21c1
DNA Barcode:
AGGACGTCACGGCCATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNAO1 (2775)
Gene Description:
G protein subunit alpha o1
Transcript:
RefSeq NM_020988.2 (NON-CURRENT)
Match location:
Position 1547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACGTCACGGCCATCATTT
Hairpin Sequence:
5'-CCGG-AGGACGTCACGGCCATCATTT-CTCGAG-AAATGATGGCCGTGACGTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACGTCACGGCCATCATTTCTCGAGAAATGATGGCCGTGACGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACGTCACGGCCATCATTTCTCGAGAAATGATGGCCGTGACGTCCT-3'

Other clones with same target sequence:

(none)