Construct: shRNA TRCN0000445030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009040.3-638s21c1
DNA Barcode:
GGGTGAAGGTGGCTATTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rdh16 (19683)
Gene Description:
retinol dehydrogenase 16
Transcript:
RefSeq NM_009040.3 (CURRENT)
Match location:
Position 638 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTGAAGGTGGCTATTATAG
Hairpin Sequence:
5'-CCGG-GGGTGAAGGTGGCTATTATAG-CTCGAG-CTATAATAGCCACCTTCACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTGAAGGTGGCTATTATAGCTCGAGCTATAATAGCCACCTTCACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTGAAGGTGGCTATTATAGCTCGAGCTATAATAGCCACCTTCACCC-3'

Other clones with same target sequence:

(none)