Construct: shRNA TRCN0000445070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_147779.1-948s21c1
DNA Barcode:
ACTCTGATCAAGCGGGTTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sftpb (20388)
Gene Description:
surfactant associated protein B
Transcript:
RefSeq NM_147779.1 (NON-CURRENT)
Match location:
Position 948 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCTGATCAAGCGGGTTCAA
Hairpin Sequence:
5'-CCGG-ACTCTGATCAAGCGGGTTCAA-CTCGAG-TTGAACCCGCTTGATCAGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCTGATCAAGCGGGTTCAACTCGAGTTGAACCCGCTTGATCAGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCTGATCAAGCGGGTTCAACTCGAGTTGAACCCGCTTGATCAGAGT-3'

Other clones with same target sequence:

(none)