Construct: shRNA TRCN0000445135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000961.3-1446s21c1
DNA Barcode:
GGAGCTGATCAACGCAGATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTGIS (5740)
Gene Description:
prostaglandin I2 synthase
Transcript:
RefSeq NM_000961.3 (NON-CURRENT)
Match location:
Position 1446 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGCTGATCAACGCAGATGT
Hairpin Sequence:
5'-CCGG-GGAGCTGATCAACGCAGATGT-CTCGAG-ACATCTGCGTTGATCAGCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGCTGATCAACGCAGATGTCTCGAGACATCTGCGTTGATCAGCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGCTGATCAACGCAGATGTCTCGAGACATCTGCGTTGATCAGCTCC-3'

Other clones with same target sequence:

(none)