Construct: shRNA TRCN0000445333

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198859.3-3003s21c1
DNA Barcode:
GCGATGAGCTGCTGCACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRICKLE2 (166336)
Gene Description:
prickle planar cell polarity protein 2
Transcript:
RefSeq NM_198859.3 (NON-CURRENT)
Match location:
Position 3003 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445333)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGAGCTGCTGCACAAAT
Hairpin Sequence:
5'-CCGG-GCGATGAGCTGCTGCACAAAT-CTCGAG-ATTTGTGCAGCAGCTCATCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGAGCTGCTGCACAAATCTCGAGATTTGTGCAGCAGCTCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGAGCTGCTGCACAAATCTCGAGATTTGTGCAGCAGCTCATCGC-3'

Other clones with same target sequence:

(none)