Construct: shRNA TRCN0000445377

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030101.1-351s21c1
DNA Barcode:
AGTGAGGCTGTCTGTTGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pgpep1l (78444)
Gene Description:
pyroglutamyl-peptidase I-like
Transcript:
RefSeq NM_030101.1 (CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445377)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGAGGCTGTCTGTTGTCAA
Hairpin Sequence:
5'-CCGG-AGTGAGGCTGTCTGTTGTCAA-CTCGAG-TTGACAACAGACAGCCTCACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGAGGCTGTCTGTTGTCAACTCGAGTTGACAACAGACAGCCTCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGAGGCTGTCTGTTGTCAACTCGAGTTGACAACAGACAGCCTCACT-3'

Other clones with same target sequence:

(none)