Construct: shRNA TRCN0000445394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010274.3-1034s21c1
DNA Barcode:
CGTGAGAGCCAAATGCGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpd2 (14571)
Gene Description:
glycerol phosphate dehydrogenase 2, mitochondrial
Transcript:
RefSeq NM_010274.3 (CURRENT)
Match location:
Position 1034 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAGAGCCAAATGCGTTAT
Hairpin Sequence:
5'-CCGG-CGTGAGAGCCAAATGCGTTAT-CTCGAG-ATAACGCATTTGGCTCTCACG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAGAGCCAAATGCGTTATCTCGAGATAACGCATTTGGCTCTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAGAGCCAAATGCGTTATCTCGAGATAACGCATTTGGCTCTCACG-3'

Other clones with same target sequence:

(none)