Construct: shRNA TRCN0000445538

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009644.2-2625s21c1
DNA Barcode:
GTGTCTCTGAGTACATCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ahrr (11624)
Gene Description:
aryl-hydrocarbon receptor repressor
Transcript:
RefSeq NM_009644.2 (NON-CURRENT)
Match location:
Position 2625 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445538)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCTCTGAGTACATCATAT
Hairpin Sequence:
5'-CCGG-GTGTCTCTGAGTACATCATAT-CTCGAG-ATATGATGTACTCAGAGACAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCTCTGAGTACATCATATCTCGAGATATGATGTACTCAGAGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCTCTGAGTACATCATATCTCGAGATATGATGTACTCAGAGACAC-3'

Other clones with same target sequence:

(none)