Construct: shRNA TRCN0000445607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002232.3-1554s21c1
DNA Barcode:
ACAGTGGGTTACGGCGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNA3 (3738)
Gene Description:
potassium voltage-gated channel subfamily A member 3
Transcript:
RefSeq NM_002232.3 (NON-CURRENT)
Match location:
Position 1554 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGTGGGTTACGGCGATATG
Hairpin Sequence:
5'-CCGG-ACAGTGGGTTACGGCGATATG-CTCGAG-CATATCGCCGTAACCCACTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGTGGGTTACGGCGATATGCTCGAGCATATCGCCGTAACCCACTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGTGGGTTACGGCGATATGCTCGAGCATATCGCCGTAACCCACTGT-3'

Other clones with same target sequence:

(none)