Construct: shRNA TRCN0000445625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011012.4-703s21c1
DNA Barcode:
AGTAAAGCCCAGGCCGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oprl1 (18389)
Gene Description:
opioid receptor-like 1
Transcript:
RefSeq NM_011012.4 (NON-CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAAAGCCCAGGCCGTTAAT
Hairpin Sequence:
5'-CCGG-AGTAAAGCCCAGGCCGTTAAT-CTCGAG-ATTAACGGCCTGGGCTTTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAAAGCCCAGGCCGTTAATCTCGAGATTAACGGCCTGGGCTTTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAAAGCCCAGGCCGTTAATCTCGAGATTAACGGCCTGGGCTTTACT-3'

Other clones with same target sequence:

(none)