Construct: shRNA TRCN0000445662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207303.2-1830s21c1
DNA Barcode:
TATGTTCATGGAGGGTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATRNL1 (26033)
Gene Description:
attractin like 1
Transcript:
RefSeq NM_207303.2 (NON-CURRENT)
Match location:
Position 1830 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGTTCATGGAGGGTATAAA
Hairpin Sequence:
5'-CCGG-TATGTTCATGGAGGGTATAAA-CTCGAG-TTTATACCCTCCATGAACATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGTTCATGGAGGGTATAAACTCGAGTTTATACCCTCCATGAACATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGTTCATGGAGGGTATAAACTCGAGTTTATACCCTCCATGAACATA-3'

Other clones with same target sequence:

(none)