Construct: shRNA TRCN0000445917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178680.4-1051s21c1
DNA Barcode:
ATGACAACTCACGCACCATCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Unc45b (217012)
Gene Description:
unc-45 myosin chaperone B
Transcript:
RefSeq NM_178680.4 (CURRENT)
Match location:
Position 1051 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACAACTCACGCACCATCT
Hairpin Sequence:
5'-CCGG-ATGACAACTCACGCACCATCT-CTCGAG-AGATGGTGCGTGAGTTGTCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACAACTCACGCACCATCTCTCGAGAGATGGTGCGTGAGTTGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACAACTCACGCACCATCTCTCGAGAGATGGTGCGTGAGTTGTCAT-3'

Other clones with same target sequence:

(none)