Construct: shRNA TRCN0000445946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198482.1-340s21c1
DNA Barcode:
TGCTGACTCAGCCTTGGTACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH2D6 (284948)
Gene Description:
SH2 domain containing 6
Transcript:
RefSeq NM_198482.1 (NON-CURRENT)
Match location:
Position 340 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGACTCAGCCTTGGTACT
Hairpin Sequence:
5'-CCGG-TGCTGACTCAGCCTTGGTACT-CTCGAG-AGTACCAAGGCTGAGTCAGCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGACTCAGCCTTGGTACTCTCGAGAGTACCAAGGCTGAGTCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGACTCAGCCTTGGTACTCTCGAGAGTACCAAGGCTGAGTCAGCA-3'

Other clones with same target sequence:

(none)