Construct: shRNA TRCN0000446028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015374.1-2453s21c1
DNA Barcode:
GCTCTGACTTCTAGGAGCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUN2 (25777)
Gene Description:
Sad1 and UNC84 domain containing 2
Transcript:
RefSeq NM_015374.1 (NON-CURRENT)
Match location:
Position 2453 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTGACTTCTAGGAGCACA
Hairpin Sequence:
5'-CCGG-GCTCTGACTTCTAGGAGCACA-CTCGAG-TGTGCTCCTAGAAGTCAGAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTGACTTCTAGGAGCACACTCGAGTGTGCTCCTAGAAGTCAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTGACTTCTAGGAGCACACTCGAGTGTGCTCCTAGAAGTCAGAGC-3'

Other clones with same target sequence:

(none)