Construct: shRNA TRCN0000446231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001720.3-1696s21c1
DNA Barcode:
GACCCTCACAACCACGTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMP8B (656)
Gene Description:
bone morphogenetic protein 8b
Transcript:
RefSeq NM_001720.3 (NON-CURRENT)
Match location:
Position 1696 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCTCACAACCACGTACAT
Hairpin Sequence:
5'-CCGG-GACCCTCACAACCACGTACAT-CTCGAG-ATGTACGTGGTTGTGAGGGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCTCACAACCACGTACATCTCGAGATGTACGTGGTTGTGAGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCTCACAACCACGTACATCTCGAGATGTACGTGGTTGTGAGGGTC-3'

Other clones with same target sequence:

(none)