Construct: shRNA TRCN0000446419

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017766.3-1304s21c1
DNA Barcode:
GAACGGGAGCACCTACAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CASZ1 (54897)
Gene Description:
castor zinc finger 1
Transcript:
RefSeq NM_017766.3 (NON-CURRENT)
Match location:
Position 1304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446419)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGGGAGCACCTACAAGAA
Hairpin Sequence:
5'-CCGG-GAACGGGAGCACCTACAAGAA-CTCGAG-TTCTTGTAGGTGCTCCCGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGGGAGCACCTACAAGAACTCGAGTTCTTGTAGGTGCTCCCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGGGAGCACCTACAAGAACTCGAGTTCTTGTAGGTGCTCCCGTTC-3'

Other clones with same target sequence:

(none)