Construct: shRNA TRCN0000446484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005183.2-4289s21c1
DNA Barcode:
GTTTACCTGTGGTAGCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CACNA1F (778)
Gene Description:
calcium voltage-gated channel subunit alpha1 F
Transcript:
RefSeq NM_005183.2 (NON-CURRENT)
Match location:
Position 4289 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTACCTGTGGTAGCAATTT
Hairpin Sequence:
5'-CCGG-GTTTACCTGTGGTAGCAATTT-CTCGAG-AAATTGCTACCACAGGTAAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTACCTGTGGTAGCAATTTCTCGAGAAATTGCTACCACAGGTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTACCTGTGGTAGCAATTTCTCGAGAAATTGCTACCACAGGTAAAC-3'

Other clones with same target sequence:

(none)