Construct: shRNA TRCN0000446673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013869.5-2112s21c1
DNA Barcode:
TGAACCAAACTGACAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf19 (29820)
Gene Description:
tumor necrosis factor receptor superfamily, member 19
Transcript:
RefSeq NM_013869.5 (CURRENT)
Match location:
Position 2112 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAACCAAACTGACAACATTT
Hairpin Sequence:
5'-CCGG-TGAACCAAACTGACAACATTT-CTCGAG-AAATGTTGTCAGTTTGGTTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAACCAAACTGACAACATTTCTCGAGAAATGTTGTCAGTTTGGTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAACCAAACTGACAACATTTCTCGAGAAATGTTGTCAGTTTGGTTCA-3'

Other clones with same target sequence:

(none)