Construct: shRNA TRCN0000447221

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002045.3-477s21c1
DNA Barcode:
GCTCATAAGGCCGCAACCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAP43 (2596)
Gene Description:
growth associated protein 43
Transcript:
RefSeq NM_002045.3 (NON-CURRENT)
Match location:
Position 477 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000447221)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCATAAGGCCGCAACCAAA
Hairpin Sequence:
5'-CCCG-GCTCATAAGGCCGCAACCAAA-CTCGAG-TTTGGTTGCGGCCTTATGAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCATAAGGCCGCAACCAAACTCGAGTTTGGTTGCGGCCTTATGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCATAAGGCCGCAACCAAACTCGAGTTTGGTTGCGGCCTTATGAGC-3'

Other clones with same target sequence:

(none)